Laboratory Exercises in Microbiology – Lansing M. Prescott, John P. Harley – 5th Edition

1 Microscopic Techniques
1 Bright-Field Light Microscope and Microscopic Measurement of Organisms
2 The Hanging Drop Slide and Bacterial Motility
3 Dark-Field Light Microscope
4 Phase-Contrast Light Microscope
5 Flourescence Microscope
2 Bacterial Morphology and Staining
6 Negative Staining
7 Smear Preparation and Simple Staining
8 Gram Stain
9 Acid-Fast Staining (Ziehl-Neelsen and Kinyoun) Procedures
10 Endospore Staining (Schaeffer-Fulton or Wirtz-Conklin)
11 Capsule Staining
12 Flagella Staining: West and Difco's SpotTest Methods
3 Basic Laboratory and Culture Techniques
13 Microbiological Culture Media Preparation and Sterilization
14 Culture Transfer Instruments, Techniques, and Isolation and Maintenance of Pure Cultures
15 Spread-Plate Technique
16 The Streak-Plate Technique and Different Media
17 Pour-Plate Technique
18 Cultivation of Anaerobic Bacteria
19 Determination of Bacterial Numbers
4 Biochemical Activities of Bacteria
20 Carbohydrates I: Fermentation and B-Galactosidase Activity
21 Carbohydrates II: Triple Sugar Iron Agar Tests
22 Carbohydrates III: Starch Hydrolysis
23 Lipids: Lipid Hydrolysis
24 Proteins, Amino Acids, and Enzymes I: Hydrogen Sulfide Production and Motility
25 Proteins, Amino Acids, and Enzymes II: The IMViC Tests
26 Proteins, Amino Acids, and Enzymes III: Casein Hydrolysis
27 Proteins, Amino Acids, and Enzymes IV: Gelatin Hydrolysis
28 Proteins, Amino Acids, and Enzymes V: Catalase Activity
29 Proteins, Amino Acids, and Enzymes VI: Coagulase and DNase Activity
30 Proteins, Amino Acids, and Enzymes VII:Oxidase Test
31 Proteins, Amino Acids, and Enzymes VIII: Urease Activity
32 Proteins, Amino Acids, and Enzymes IX: Lysine and Ornithine Decarboxylase Test
33 Proteins, Amino Acids, and Enzymes X: Phenylalanine Deamination
34 Proteins, Amino Acids, and Enzymes XI: Nitrate Reduction
5 Rapid Multitest Systems
35 The API 20E System
36 The Enterotube II System
6 Unknown Identification
37 Using the First Edition of Bergey's Manual of Systematic Bacteriology to Identify Bacteria
38 General Unknown
7 Environmental Factors Affecting Growth of Microorganisms
39 Temperature
40 pH
41 Osmotic Pressure
42 The Effects of Chemical Agents on Bacteria I: Disinfectants
43 The Effects of Chemical Agents on Bacteria II: Antimicrobial Agents (Kirby-Bauer Method)
44 Hand Washing, Environmental Sampling, and Microbiological Monitoring
45 Determination of a Bacterial Growth Curve: Classical and Two-Hour Methods
8 Environmental and Food Microbiology
46 Standard Coliform Most Probably Number (MPN)Test and Presence-Absence Coliform Test
47 Membrane Filter Technique for Coliform and Fecal Streptococci; KONFIRM Test for Fecal Coliforms
48 Isolation of Escherichia coli Bacteriophages from Sewage and Determining Bacteriophage Titers
49 Enumeration of Soil Microorganisms
50 Bacterial Count of a Food Product
51 Examination of Milk for Bacteria
9 Medical Microbiology
52 Agglutination Reactions: Blood Groups
53 Isolation of Normal Microbiota from the Human Body
54 Staphylococci
55 Pneumococci
56 Streptococci
57 Neisseriae
58 Aerobic and Anaerobic Endospore-Forming Bacteria
10 Survey of Selected Eucaryotic Microorganisms
59 Fungi I: Yeasts
60 Fungi II: Phycomycetes, Ascomycetes, and Basidiomycetes
11 Microbial Genetics and Genomics
61 Bacterial Mutation
62 Bacterial Transformation
63 Bacterial Conjugation: The Transfer of Antibiotic-Resistant Plasmids
64 Isolation of Genomic DNA from Saccharomyces cerevisiae
65 Isolation and Purification of Genomic DNA from Escherichia coli
66 Identifying Archaea and Bacteria Using the Internet and Computer-Assisted Gene Analysis

Appendix A Dilutions with Sample Problems
Appendix B Metric and English Measurement Equivalents
Appendix C Transmission-Absorbance Table for Spectrophotometry
Appendix D Logarithms
Appendix E pH and pH Indicators
Appendix F Scientific Notation
Appendix G Identification Charts
Appendix H Reagents, Solutions, Stains, and Tests
Appendix I Culture Media
Appendix J Sources and Maintenance of Microbiological Stock Cultures