Microscopy Techniques (Advances in Biochemical Engineering – Biotechnology) – Atsushi Miyawaki, Takeharu Nagai, Hideaki Mizuno


Light Microscopy has returned to fashion through the synergy of green fluorescent protein and a clutch of techniques involving some permutation of lasers, mathematics and acronyms, which offer the prospect of watching biological molecules at work inside living cells. This volume provides an introduction to these techniques. Will be a longer-term reference for anyone who wants to understand how these methods actually work. In this respect it is excellent.

Advances in microscopy techniques have recently had a tremendous impact on research in biochemistry and molecular biology. While the study of biochemical reactions was formerly confined to cuvette-based measurements of purified biomolecules, it is now possible for investigators to make such analyses inside the complex environment of living cells and organisms.

While some of these advanced techniques were in fact developed some time ago, it is only now that they are becoming available to a wider range of researchers as components of commercial equipment. As a consequence, there is a strong demand for the ability to help individuals from a biomedical, rather than from an instrumental background, in order for them to make the best use of these tools in their research.

To meet these requirements, the contributions in this book mainly stem from instructors present at a series of very successful training courses held in the Advanced Light Microscopy Facility at the European Molecular Biology Laboratory over the past 5 years.

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  • 1. Engineering Fluorescent Proteins
    2. Higher Harmonic Generation Microscopy
    3. Live Cell Spinning Disk Microscopy
    4. Total Internal Reflection Fluorescence Microscopy in Single Molecule Nanobioscience
    5. Fluorescence Correlation Spectroscopy with Autofluorescent Proteins
    6. Fluorescence Lifetime Imaging Microscopy (FLIM)
    7. Fluorescence Recovery after Photobleaching: Application to Nuclear Proteins
    8. Deconvolution Microscopy
    9. Spectral Imaging and Linear Unmixing in Light Microscopy
    10. Tracking Movement in Cell Biology

  • Citation
    • Full Title: Microscopy Techniques (Advances in Biochemical Engineering
    • Author/s:
    • ISBN-10: 3540236988
    • ISBN-13: 9783540315452
    • Edition: 1st Edition
    • Publication date: 2005
    • Topic: Chemistry
    • Subtopic: Analytical Chemistry
    • File Type: eBook
    • Idioma: English

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